Taken together, PRC2-associated HOTAIR inhibits the expression of WIF-1 by increasing trimethylation at H3K27 in the WIF-1 promoter region and then activates the Wnt/β-catenin signaling pathway and manifests the cell proliferation, migration, and invasion of ESCC cells (Figure 4). Here, HOTAIR is linked to esophageal squamous cell carcinoma.