Multiple biomarkers were measured in subgroups of the total study population and have been published previously (now available for the current dataset in Table S1),11,19–21 showing in the current dataset significant differences in calprotectin, chromogranin A and human β-defensin 2 in fecal samples, plasma cytokines IL-1 and IL-10/12 ratio, and visceral sensitivity by rectal barostat between IBS and HC. Here, CHGA is linked to irritable bowel syndrome.