LEP and obesity due to melanocortin 4 receptor deficiency: There is evidence that an acute lipid infusion for 24 h or exposure to a HFD over 8 – 20 weeks induces markers of inflammation in the hypothalamic NPY/AgRP neurons, which may contribute to a significant alteration in NPY/AgRP expression or content (101) and also, 6 days of exposure to a high-fat diet can induce leptin resistance in mice with a predisposition to obesity (102).