The functional significance of altered expression levels of ALKBH5 was comprehensively investigated in various cancers in regard to prognosis, genetic alterations, expression levels of ALKBH5-related genes, tumor immunity, and the lncRNA-miRNA network regulating ALKBH5 expression. We also specifically analyzed the relationship between ALKBH5 expression and the growth and progression of gliomas using both in vitro and in vivo models. Here, ALKBH5 is linked to central nervous system cancer.