Furthermore, compared to parental CAR-T, hNELFB-expressing CAR-T conferred more tumor infiltration of CD8+ and CD4+ T cells (Supplementary Fig. 8f, g), and higher memory marker CD127 expression and fewer cells with the exhaustion markers TIM3+CD39+ in both CD8+ and CD4+ populations (Supplementary Fig. 8h–k). The gene discussed is HAVCR2; the disease is neoplasm.