It was revealed that co-transfection with sh-KCNQ1OT1 and miR-124-3p inhibitor attenuated the inhibitory effect of sh-KCNQ1OT1 on the proliferation and the expression levels of α-SMA and Fibronectin of HK-2 cells, suggesting that KCNQ1OT1 interacted with miR-124-3p and inhibited miR-124-3p expression, thus regulating the occurrence and development of renal fibrosis. This evidence concerns the gene FN1 and renal fibrosis.