SNCA and Parkinson disease: First, using reverse-transcription polymerase chain reaction (RT-PCR) and immunoblotting, we confirmed that the mRNA and protein levels of SIRT2 were not altered in the SNpc in the MPTP-induced (WT mice) (Fig. 2a–d) and α-synuclein-A30P*A53T overexpression (transgenic mice) models of PD (Fig. 2e–h).