CASP8 and chronic myelogenous leukemia, BCR-ABL1 positive: Interestingly, 2-DG seems to exert its cytotoxic effect against CML cells mainly by induction of autophagy without significant apoptosis, as evidenced by an induction of LC3A-II expression, the lack of annexin-V/PI staining, and no cleavage of caspase-8 and PARP.