Of note, TMB was strongly correlated with nearly all the numbers of mutated members in the pre-specified pathways (marked by a purple arrow, Fig. 2), including the PI3K-AKT-mTOR pathway, rather than DTICs (marked by a blue arrow, Fig. 2), consistent with previous results in MSI-H CRC [14, 36]. This evidence concerns the gene AKT1 and colorectal carcinoma.