We have previously shown that CG8421 causes a high degree of colitis in IL-10−/− mice that is consistent with its high Type1 and 17 cytokine elicitation characteristic.20 Consistent with lack of ganglioside presentation on its surface,34 Siglec-1 blockade did not affect cytokine elicitation (Figures 4(c, d-e)) or uptake (Figure 4(f)) of CG8421 in whole and adherent splenocytes, respectively. This evidence concerns the gene IL10 and colitis.