The exogenous application of rMFG-E8 to A10 VSMCs significantly enhanced the Smad2 phosphorylation (Fig. 6h, i), Runx2 (Fig. 6h, j), and BMP-2 expression (Fig. 6k, l), and calcium deposition (Fig. 6m, n) induced by OM. This evidence concerns the gene SMAD2 and ocular melanoma.