LCN2 and ischemic stroke: To determine whether ER stress-dependent mechanisms play a role in regulating astrocytic LCN2 expression in ischemic stroke, we first tested whether Tg differently induced ER stress responses and LCN2 expression in primary astrocyte cultutres isolated from wild-type (Nhe1+/+) and global Nhe1 KO (Nhe1−/−) mice.