CD4 and neoplasm: To elucidate the mechanism of activity induced by D-NPDOX/C-NPNLG&BLZ-mediated cocktail chemoimmunotherapy, we first examined the frequency of the T cell subpopulation in tumor tissues, which were collected at the end of treatment in Fig. 6c to measure tumor-infiltrating CD8+ T cells (CD3+CD4-CD8+) and immunosuppressive regulatory T cells (Tregs, CD3+CD4+Foxp3+) by flow cytometry (Fig. 7a, b).