NNMT overexpression reduced DNA damage in stressed ACHN, 786-O, and 769-P cells as evidenced by the levels of γ-H2AX detected using immunofluorescence staining (Fig. 6g) and western blotting (Fig. 6h) and by DNA damage detection using the comet assay (Fig. 6i), in cultured renal cancer cell lines. The gene discussed is H2AX; the disease is renal carcinoma.