BRAF and melanoma: The melanoma cell line A375-IVLuc (Kucerova et al, 2014) continuously expressing luciferase vector pGL4.50 (luc2/CMV/Hygro) (Promega) was cultured in the presence of vemurafenib—an inhibitor of the constitutively active B-Raf V600E proto-oncogene, used for the treatment of late-stage melanoma (Flaherty et al, 2010; Chapman et al, 2011).