LIF and breast carcinoma: We further determined the levels of Glut1 on the PM in breast cancer cells by Western-blot analysis of the fraction of the PM isolated from cells, and found that ectopic LIF expression promoted endogenous Glut1 PM translocation but did not change total Glut1 protein levels in MCF7, MDA-MB 231 and T47D cells (Fig. 4B).