To further verify the reliability of the results obtained by WGCNA combined with DAVID and explore the potential mechanism by which LRRN4 regulated, we measured the expression of Akt, p-Akt, ERK 1/2 and p-ERK1/2, the downstream of the Ras signaling pathway, one of the most important mechanism of CRC development. The gene discussed is MAPK3; the disease is colorectal carcinoma.