Interestingly also, the gene encoding for O6-Methylguanine-DNA-methyltransferase (MGMT), a DNA repair enzyme known to remove alkylating modifications from DNA thereby counteracting the anti-tumorigenic effect of temozolomide (TMZ) [31], was in principal downregulated in GBM cells as compared to normal astrocytes, and in several GBM cell lines it was not detectable at all, both on mRNA and protein level (Fig. 1A and C). Here, LIG4 is linked to glioblastoma.