CCK-8 and EdU assay showed that rescue of CTSS partially restored the viability of MEOX2-silenced glioma cells (Fig. 8B, D and Fig. S7B), and limiting dilution assay revealed that rescue of CTSS also partially restored the self-renewal capacity of MEOX2-depleted GSC23 cells (Fig. 8C and Fig. S7A). This evidence concerns the gene MEOX2 and central nervous system cancer.