Previously, a retrospectively study on blood TMB (bTMB) was conducted using pretreated NSCLC patients from the OAK and POPLAR studies; the study revealed that there was a positive correlation between bTMB and tissue TMB score and that bTMB could reliably identify patients who had significant clinical improvements in PFS due to anti-PD-L1 therapy as a second-line or higher treatment in NSCLC [82]. The gene discussed is CD274; the disease is non-small cell lung carcinoma.