This estimate focuses on the diagnosis of LS in patients with CRC who underwent genetic testing in conjunction with the current tumor screen and does not include two LS cases noted above: (1) the individual with a positive IHC screen who had received a prior diagnosis of LS and did not undergo genetic testing again and (2) the individual who had a positive IHC screen and BRAF V600E was present on reflex testing but was diagnosed with LS following genetic testing. This evidence concerns the gene BRAF and Leigh syndrome.