To elucidate the biological functions of SQSTM1, GDF9, LINC01125, PTGS2, GVINP1, and TMEM64 in breast tumor cells, we knocked down the expression of the 6-gene signature using shRNA or the negative control in MCF-7 cell lines to assess cell proliferation, migration and invasion in vitro. Here, SQSTM1 is linked to breast neoplasm.