The results demonstrated that six hub genes not only were upregulated in GBM tissue samples (all p < 0.05), but also were markedly related to worse prognosis of GBM patients, including IFI30, HLA-DMA, P4HB, RCN1, FN1 and PYCARD (all p < 0.05, Fig. 4). This evidence concerns the gene P4HB and glioblastoma.