These data are relevant because it has been shown that 69% of patients who underwent chemotherapy (Bu or other chemotherapies such as melphalan) before hematopoietic stem cell transplantation had an increased risk of persistent azoospermia.[54] The present work work helps to further understand fertility disorders in the context of cancer treatments and provides new perspectives for developing therapeutic solutions targeting TGR5 for fertility disorders to promote germ cell lineage regeneration. This evidence concerns the gene GPBAR1 and Azoospermia.