Accumulative evidences have demonstrated that TLRs’ stimulation leads to the activation of multiple signaling pathways, including NF-κB, MAPK, Jun N-terminal kinases, P38, and extracellular signal-regulated kinase, as well as several interferon regulatory factors, which result in activation of inflammatory cytokines implicated in hepatocellular carcinoma. This evidence concerns the gene NFKB1 and hepatocellular carcinoma.