ANXA5 and central nervous system cancer: To study whether the decrease in the number of human glioma cell lines after TAC1R siRNA gene silencing was due to an inhibition of the cell proliferation or to an induction of apoptotic or necrotic cell death mechanisms, the number of apoptotic and necrotic cells after transfection by flow cytometer using an Annexin V- conjugated with fluorescein isothiocyanate (FITC) kit was assessed.