When compared with controls, rats with NAFLD had significantly elevated red cell distribution width (P = 0.041), ALT (P = 0.009), spleen diameter (P < 0.001), portal venous pressure (P < 0.001), LSM (P < 0.001), and SSM (P < 0.001). The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.