We compared the therapeutic effects and found that VMT had almost consistent inhibitory ectopic lesion progression effect in mice like LA, along with inflammatory cytokines reduced and key proteins of NF-κB signaling pathway downregulated, suggesting that vaginal bacteria play an important role in endometriotic lesion progression and reconstructing the vaginal microbiota microenvironment to normal could effectively reduce the inflammatory level of endometriosis mice. Here, NFKB1 is linked to endometriosis.