In this study, we identified two novel homozygous variants (c.487T > C [p.Cys163Arg], and c.1425G > A [p.Trp475*]) in PADI6 in two infertile patients from a cohort of 75 females with the phenotype of early embryonic arrest, which caused recurrent ART failure. Here, PADI6 is linked to Infertility.