To investigate whether Juglone affects the colony forming potential of CD44+CD133+ tumor-initiating Caco-2 cells, FACS-isolated CD44+CD133+ tumor-initiating Caco-2 cells and ΔCD44+CD133+ non-tumor-initiating Caco-2 cells were treated with different amounts of Juglone. This evidence concerns the gene PROM1 and neoplasm.