To investigate whether Juglone or KPT6566 can eliminate tumor-initiating cells in CRC, Caco-2 cells were divided into a tumor-initiating subpopulation (CD44+CD133+-positive Caco-2 cells) and a non-tumor-initiating subpopulation (CD44+CD133+-negative Caco-2 cells; tumor-initiating cell-depleted Caco-2 cells). This evidence concerns the gene PROM1 and colorectal carcinoma.