TGFB1 and hydrops fetalis: Fiedler et al. (2019) developed a myocardial ischemia model exhibiting the most prevalent etiology of HF and evaluated optimized analogs by screening for cardioprotective activity. Iseoka et al. induced an HF model by TGF-β stimulation and detected anti-fibrotic drugs that decreased fibrotic ECM expression and improved contraction and relaxation using a cell-motion system (Iseoka et al., 2021).