CRISPR-CasRx system was incorporated in glial cells, in vivo and targeted the single RNA-binding protein, polypyrimidine tract-binding protein 1 for the conversion of glia to neuron using CasRx -mediated knockdown of Ptbp1 shows a potential therapeutic approach for tackling neurodegenerative diseases and brain damage due to neuronal loss. Here, PTBP1 is linked to neurodegenerative disease.