MISP and cancer: This study explored the role of MISP in PDAC through basic experiments in cell lines and integrating the data from TCGA, GEO, Human Protein Atlas (HPA), Gene Expression Profiling Interactive Analysis 2 (GEPIA 2), Kaplan-Meier Plotter, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Tumor Immune Estimation Resource 2.0 (TIMER2.0), and The Cancer Immunome Atlas (TCIA) through bioinformatics analysis.