We analyzed NRAGE expression in public database, and found that it was upregulated both in ESCA samples (182 cases) compared with adjacent normal tissue samples (286 cases) (p <0.05) (match TCGA normal and GTEx data, http://gepia.cancer-pku.cn/) and in ESCC samples compared with paired Paracancerous tissue from the GSE20347 dataset (p = 0.0001, https://www.ncbi.nlm.nih.gov/geo/) (Figure 6A). The gene discussed is MAGED1; the disease is esophageal squamous cell carcinoma.