Consistent with the results of wound healing assay, western blotting analyses revealed upregulated expression of E-cadherin and downregulated expression of N-cadherin and vimentin (Figures 3C–F) by treatment with U50488h or oxycodone, indicating that U50488h or oxycodone suppressed the epithelial-mesenchymal transition (EMT) of the HCC cells. The gene discussed is VIM; the disease is hepatocellular carcinoma.