We further observed differential expression of the nineteen pyroptosis-related genes using vioplot, of which seventeen pyroptosis-related genes were more highly expressed in glioma tissues, including IRAK4, RELB, HMOX1, TP53, TLR4, IL18, GBP1, GBP2, GBP3, CASP1, AOAH, IRF8, PYCARD, TRAF6, DDX58, TIGAR and PANX1, while expression of GSMB and ADCY4 was higher in normal tissues (Figure 1C). The gene discussed is TLR4; the disease is glioma.