The 10 crucial pathways that may be regulated by PCRR in the treatment of ALF by the KEGG pathway enrichment analysis included pathway in cancer, AGE-RAGE pathway in diabetic complications, hepatitis B, prostate cancer, bladder cancer, fluid shear stress and atherosclerosis, IL-17 pathway, Kaposi sarcoma-associated herpesvirus infection, pancreatic cancer, and TNF. The gene discussed is TNF; the disease is cancer.