The top 10 ALF-related signaling pathways were identified as pathway in cancer (path:hsa05200), AGE-RAGE signaling pathway in diabetic complications (path:hsa04933), hepatitis B (path:hsa05161), prostate cancer (path:hsa05215), bladder cancer (path:hsa05219), fluid shear stress and atherosclerosis (path:hsa05418), interlukin (IL)-17 signaling pathway (path:hsa04657), Kaposi sarcoma-associated herpesvirus infection (path:hsa05167), pancreatic cancer (path:hsa05212), and tumor necrosis factor (TNF) signaling pathway (path:hsa04668). This evidence concerns the gene TNF and familial pancreatic carcinoma.