In this study, we collected and detected the expression level of miR-372-3p in 153 frozen tissue samples (including 66 colon cancer tissues, matched with 66 normal and 21 adjacent tissues), and then analyzed the correlation between miR-372-3p expression levels with pathological immunohistochemical (IHC) staining parameters, including tumor-suppressor gene p53, mutL homolog 1 (MLH1), mutS homolog 2 (MSH2), mutS homolog 6 (MSH6), mismatch repair system component 2 (PMS2), marker of proliferation Ki-67 (Ki67) and hypoxia response element-2 (HRE-2) and several potential miR-372-3p target genes. This evidence concerns the gene MSH6 and malignant colon neoplasm.