RB1 and cancer: Similarly, Francis et al. using clinical next-generation sequencing found additional molecular alterations in all study samples (no two specimens had identical genetic pattern), being the most common non-RB1 gene alteration BCOR, which was associated with poor metastases-free survival (MFS), while vitreous seeding was correlated with 16q loss and 1q gains; and in 11% of cases non-RB1 germline mutations in other cancer-associated genes were found (Francis et al., 2021).