Among the 505 participants in the final analysis, 2 participants (0.39%) presented indeterminate result in CXCL10 mRNA release assay, due to invalid ΔCT in the mitogen tube (>-1.2), while 7 participants (1.39%) presented an indeterminate result in T-SPOT.TB, due to higher SFCs (>10 spots) in Nil control well or lower SFCs (<20 spots) in positive control well. The gene discussed is CXCL10; the disease is tuberculosis.