Five hemizygous missense AFF2 variants were identified in five unrelated individuals with partial epilepsy, including c.230A > T/p.N77I, c.391C > T/p.H131Y, c.1540C > T/p.R514C, c.2009G > A/p.R670H, and c.2074C > G/p.P692A. This evidence concerns the gene AFF2 and focal epilepsy.