RNF8 and malignant colon neoplasm: Although we have reported in our previous study that RNF8 is an activator for MYC transcription via regulating β-catenin nuclear translocation in WNT/APC constructively activated colon cancer (CCR) cells [39], c-Myc mRNA and protein levels are not changed substantially in RNF8 silencing PC cells (Supplementary Fig. 5A–C), which may be due to the different activation extent of WNT signaling between CCR and PC cells.