To determine the main isoform of IL-32 in ESCC tumor tissues and ESCC cell lines, we firstly used qRT-PCR to detect the expression of all IL-32 isoforms in ESCC cell lines (KYSE150, KYSE450, EC1, EC109 and EC9706) and the normal esophagus epithelial Het-1 A cells (Fig. S2), and found that IL-32 was highly expressed in EC109. The gene discussed is IL32; the disease is neoplasm.