A total of 34 factors were found to be significantly different between DF and SD, age, diabetes history, secondary infection, seroDENV-2/3, bleeding, vomiting, ascites, pleural effusion, lethargy and petechiae, were positive associated with SD; HCT, ALT, AST, CK, BUN, LDH, IL-10, IL-8, sVCAM-1, and IP-10 were increasing but total protein, albumin and PLT were decreasing in level during SD. The gene discussed is GPT; the disease is Pleural effusion.