For validating the five hub genes related to survival, we detected the expression difference of these five genes in 18 pairs of cancer and adjacent tissues by RT-PCR, and the results showed that the expression of ADRA1B mRNA in the adjacent tissues was higher than that in the adjacent tissues, while the expression of BRS3, CALCA, and CALCR mRNA in the cancer tissues was significantly higher than that in the adjacent tissues. The gene discussed is CALCA; the disease is cancer.