To examine whether rs7158663 and rs322931 polymorphisms correlated with the expression of TNF-α, IL-1β and IL-6, ELISA was carried out to compare the abundance of TNF-α, IL-1β and IL-6 protein in the peripheral blood of Crohn’s disease patients carrying different genotypes at rs7158663 and rs322931. This evidence concerns the gene TNF and Crohn disease.