In this study, by recruiting patients with Crohn’s disease, we compared the parameters including MEG3, miR-181b, and TNF expression as well as the levels of inflammatory factors in patients carrying different genotype of MEG3 and miR-181b, so as to study the correlation between the SNPs and MEG3/miR-181b expression as well as the severity of anal abscess in patients with Crohn’s disease. This evidence concerns the gene TNF and Crohn disease.