HMGCS2 and steatosis: Specifically, early weaning significantly removed lipid droplets and likely reduced subsequent lipid accumulation at the center of the liver in Hmgcs2-KO mice, while some lipid droplets remained at the periphery of the liver, mainly in the form of macrovesicular steatosis (i.e., large lipid droplets displacing nuclei within hepatocytes) (Supplementary Fig. 3D).