H&E staining and Perilipin 2 (Plin2) immunostaining of liver sections confirmed HFD-induced severe hepatosteatosis, as these mice showed hypertrophied hepatocytes with significant accumulation of lipids, particularly microvesicular steatosis, associated with advanced NAFLD and characterized by the build-up of numerous small lipid droplets surrounding centrally located nuclei within hepatocytes [44] (Figure 1A). Here, PLIN2 is linked to metabolic dysfunction-associated steatotic liver disease.