Thus, to drive ketogenesis in vivo, we intravenously injected HFD-induced NAFLD mice with adenovirus overexpressing human HMGCS2 (Ad-HMGCS2) (Figure 5A), which has a 95% protein sequence homology to mouse Hmgcs2. A GFP-expressing adenovirus (Ad-GFP) was used for control mice. Here, HMGCS2 is linked to metabolic dysfunction-associated steatotic liver disease.