Indeed, the depletion of myosin Vb, syntaxin-3, or syntaxin-binding protein-2 in Caco-2 cells faithfully reproduced the key in vivo phenotypes of intestinal epithelial cell in MVID patients resulting from mutations in MYO5B,12,13,19,27,36STX3,20,37 and STXBP2,18,37,38 with equal performance as human and mouse 3D enteroids.20 Here, STXBP2 is linked to microvillus inclusion disease.