GPHN and infection: eGFP, mCherry, mEos2 and other fluorescent proteins can be fused with gephyrin and expressed in cells via transfection or infection.[6, 13, 14] Fluorescent gephyrin chimeras have been successfully used for synapse studies both in fixed and live cell cultures,[6, 13, 14] as well as in conditional expression in transgenic animals,[15] but an inherent drawback of induced secondary gephyrin expression are morphological and/or functional effects in cells.[15, 16, 17] To circumvent these drawbacks, two alternative methods of genetic tagging were applied.