Using coculture approaches with mouse fibroblast (3T3) and alveolar cells murine lung epithelial-12 (MLE12), we demonstrate that the SIX1-mediated effects on cell proliferation and expression levels of α-SMA and Col1a1 are reduced by ISO-1, a MIF inhibitor, providing further evidence for the SIX1-MIF link in lung fibrosis. Here, ACTA1 is linked to pulmonary fibrosis.